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. 2023 Jul 18;14:4297. doi: 10.1038/s41467-023-40086-3

Fig. 5. HCK knockout globally and in myeloid cell ameliorated kidney fibrosis in a murine UUO model.

Fig. 5

A Schematic diagram shows the strategy of loxP-HCK knock-out mice developed in EuMMCR. B Western blot demonstrated HCK global (CMV) and myeloid cell specific (LYZ2) KO in BMDM from mice. C mRNA levels of pro-fibrotic markers were decreased in HCK global and myeloid cell KO UUO kidneys compared to WT mice. D Representative Masson’s trichrome staining images from sham operated & UUO kidneys of WT, CMV KO and LYZ2 KO mice at 7 days post-UUO. E Morphometric quantification (n = 5 animals; 5 random fields/animal) of the fibrosis positive area for Masson stain. F Immunofluorescence (IF) stain indicated F4/80 positive macrophage and HCK both dramatically decreased in HCK KO UUO kidneys. G Western blot to show M1 and M2 macrophage markers and quantification of band intensity and adjusted by macrophage number in UUO kidneys. H Representative images and quantification (n = 5 animals; 5 random fields/animal) of IF staining for EdU positive F4/80 macrophages in UUO kidneys. (C, E, F, G and H) *p < 0.05. Source data are provided as a Source Data file.