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. 2023 Jul 18;14:4297. doi: 10.1038/s41467-023-40086-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Western blot to show BT424 regulation of autophagy (A) and macrophage polarization (B) in Raw264.7 macrophage cell line. C MTT assay was tested for BT424 treatment with gradient dosages for cell proliferation in Raw264.7. D Representative of flow cytometric image and quantification for propidium Iodide (PI) stain to measure cell cycle for control and BT424 treatment in Raw264.7 cells. E Click-iT™ EdU cell proliferation assay and Ki67 IF staining (F) were performed to measure proliferation of Raw264.7 with control and BT424 treatment. G Scratch assay was performed in Raw264.7 cells for control and BT424 treatment. The yellow lines are the scratch edge of the cells generated by the software when to measure the scratch distance. C–G ^*p < 0.05, D with ANOVA test, others with t test. Baf1: bafilomycin A1. Source data are provided as a Source Data file.