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. 2023 Jul 18;14:4297. doi: 10.1038/s41467-023-40086-3

Fig. 9. Inhibition of HCK by BT424 reduced renal fibrosis process in UUO model by regulating macrophage activation and autophagy.

Fig. 9

A Representative images of H&E, COL1A1 IF and Masson trichrome stain from sham operated & UUO kidneys of BT424- or vehicle-treated mice. B Morphometric quantification (n = 5 animals; 3-4 random HPFs/animal) of the COL1A1 IF stain positive area. C mRNA levels of pro-fibrotic markers at 7 days post-UUO by qPCR. D IF stain showed F4/80 positive macrophage dramatically decreased in BT424 treated UUO kidneys. E Western blot assay demonstrated macrophage makers in 7 days post-UUO kidneys. Quantification for bands intensity and adjusted by macrophage cell numbers. F IF stain showed LC3 intensity was increased in F4/80 positive macrophage in BT424 treated UUO kidneys. B–F t test *p < 0.05. Source data are provided as a Source Data file.