Table 1.
Top-ranked proteins identified by immunoprecipitation-mass spectrometry (IP-MS) that interact with HCK, FYN and SRC
| Identified proteins (for HCK) | Coverage | calc. pI | Spectra# | Spectra# | Ratio |
|---|---|---|---|---|---|
| Tyrosine-protein kinase (HCK) | 72.62357 | 6.7 | 14 | 389 | 24.44 |
| E3 ubiquitin-protein ligase CBL (CBL) | 15.67329 | 6.54 | 0 | 10 | 6.00 |
| Autophagy-related protein 2 homolog A (ATG2A) | 5.159959 | 5.88 | 0 | 8 | 5.00 |
| Neural Wiskott-Aldrich syndrome protein (WASL) | 20.19802 | 7.93 | 0 | 6 | 4.00 |
| Guanine nucleotide-binding protein b4 (GNB4) | 32.05882 | 6 | 1 | 10 | 4.00 |
| Ras-related protein Rab-3D (RAB3D) | 25.57078 | 4.93 | 0 | 6 | 4.00 |
| Identified proteins (for FYN) | Coverage | calc. pI | Spectra# | Spectra# | Ratio |
| Tyrosine-protein kinase Fyn (FYN) | 54.93482 | 6.67 | 1 | 71 | 24.33 |
| Hsp90 co-chaperone Cdc37 (CDC37) | 30.68783 | 5.25 | 0 | 18 | 10.00 |
| Protein scribble homolog (SCRIB) | 10.2719 | 5.1 | 0 | 11 | 6.50 |
| Fatty acid synthase (FASN) | 14.01832 | 6.44 | 1 | 14 | 5.33 |
| ATP-dependent RNA helicase A (DHX9) | 18.34646 | 6.84 | 0 | 8 | 5.00 |
| Heat shock protein HSP 90-alpha (HSP90AA1) | 50.81967 | 5.02 | 9 | 53 | 5.00 |
| Identified Proteins (for SRC) | Coverage | calc. pI | Spectra# | Spectra# | Ratio |
| Proto-oncogene tyrosine-protein kinase Src (SRC) | 71.64179 | 7.42 | 6 | 1238.00 | 155.00 |
| V-type proton ATPase catalytic A (ATP6V1A) | 75.20259 | 5.52 | 0 | 96.00 | 49.00 |
| DNA-dependent protein kinase catalytic (PRKDC) | 34.4719 | 7.12 | 2 | 141.00 | 35.75 |
| V-type proton ATPase subunit B (ATP6V1B2) | 65.55773 | 5.81 | 0 | 68.00 | 35.00 |
| ATP-binding cassette sub-family D 3 (ABCD3) | 44.00607 | 9.36 | 0 | 37.00 | 19.50 |
| Probable ATP-depen RNA helicase (DDX20) | 35.43689 | 6.95 | 0 | 36 | 19.00 |