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Determination of Km values of cognate amino acid substrates and ATP of internal adenylation domains LicA1-A, LicB2-A, and LicC-A
| Tested domain | Substrate |
Km (mmol · liter−1) ± SD
|
|
|---|---|---|---|
| Amino acid | ATPa | ||
| LicA1-A | l-Gln | 1.4 ± 0.5 | 5 ± 3 |
| LicB2-A | l-Asp | 0.3 ± 0.15 | 1 ± 0.3 |
| LicC-A | l-Ile | 0.4 ± 0.15 | 0.1 ± 0.02 |
| LicC-A | l-Leu | 3 ± 1 | 5 ± 2 |
| LicC-A | l-Val | 2.5 ± 1 | 0.6 ± 0.2 |
Experiments were performed in the presence of the specific substrate amino acid.
