Table 2.
Sensitivity to phosphodiesterase inhibitor NPD-001 upon inducible RNAi.
| RNAi target | EC50 NPD-001 (Mean ± SEM), μM | Resistance factor | P-value | |
|---|---|---|---|---|
| - Tet | + Tet | |||
| parental control (n = 6) | 0.14 ± 0.01 | 0.17 ± 0.01 | 1.18 | 0.0369 |
| CARP5 (n = 9) | 0.14 ± 0.01 | 0.15 ± 0.01 | 1.09 | 0.0988 |
| CARP6 (n = 12) | 0.25 ± 0.04 | 0.61 ± 0.02 | 2.41 | <0.0001 |
| CARP7 (n = 12) | 0.22 ± 0.03 | 3.70 ± 0.27 | 16.91 | <0.0001 |
| CARP8 (n = 12) | 0.21 ± 0.01 | 0.41 ± 0.02 | 1.98 | <0.0001 |
| CARP9 (n = 12) | 0.26 ± 0.03 | 0.44 ± 0.08 | 1.72 | 0.0238 |
| CARP10 (n = 9) | 0.17 ± 0.01 | 0.30 ± 0.01 | 1.75 | <0.0001 |
| CARP11 (n = 12) | 0.18 ± 0.01 | 0.30 ± 0.01 | 1.69 | <0.0001 |
n = total number of replicates; P-value was calculated by unpaired, one-sided t-test.