FIG. 2.

Time course of cell growth, 2,4-DNP uptake, and nitrite release by Rhodococcus strain RB1 cultured with 2,4-DNP and ammonium as nitrogen sources. Cells were grown under aerobic conditions with 50 mM sodium acetate, 0.5 mM 2,4-DNP, and 2 mM ammonium chloride. At the times indicated, A₆₀₀ (•), 2,4-DNP concentration (○), and nitrite concentration (▴) were measured. The arrow indicates the time at which the concentration of ammonium in the medium was undetectable.