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Sliding window plot of the number of substitutions per 100 sites for synonymous (pS) and nonsynonymous (pN) sites between H6 (O55, DEC 2a) and H7 (O157, DEC 3a) fliC alleles. The difference (pN − pS) is a measure of the level of selective constraint on various parts of the molecule. A diagram of the five-domain model of flagellin (27) is shown at the bottom. The shaded regions denote the discontinuous coding regions predicted for the G3 domain.
