Table 2.
The developed multiplex PCR method was applied for the identification of S. Pullorum and S. Gallinarum isolates from one chicken farm.
| Serovar (no. of isolates) | Isolate no. | PCR results (torT/I137_14430/stn) | Dulcitol fermentation | Ornithine decarboxylase |
|---|---|---|---|---|
| Pullorum (10) | Ch4 | −/+/+ | – | + |
| Ch5 | −/+/+ | – | + | |
| Ch9 | −/+/+ | – | + | |
| Ch10 | −/+/+ | – | + | |
| Ch11 | −/+/+ | – | + | |
| Ch12 | −/+/+ | – | + | |
| Ch16 | −/+/+ | – | + | |
| Ch17 | −/+/+ | – | + | |
| Ch18 | −/+/+ | – | + | |
| Ch24 | −/+/+ | – | + | |
| Gallinarum (2) | Ch22 | +/−/+ | + | – |
| Ch23 | +/−/+ | + | – | |
| Enteritidis (9) | Ch1 | +/+/+ | / | / |
| Ch2 | +/+/+ | / | / | |
| Ch3 | +/+/+ | / | / | |
| Ch6 | +/+/+ | / | / | |
| Ch7 | +/+/+ | / | / | |
| Ch8 | +/+/+ | / | / | |
| Ch13 | +/+/+ | / | / | |
| Ch14 | +/+/+ | / | / | |
| Ch15 | +/+/+ | / | / | |
| Indiana (3) | Ch19 | +/+/+ | / | / |
| Ch20 | +/+/+ | / | / | |
| Ch21 | +/+/+ | / | / |
The traditional serotyping of the Salmonella isolates was determined based on the White-Kauffmann-Le Minor scheme. The traditional differentiation of Salmonella biovars Pullorum and Gallinarum was conducted with the dulcitol fermentation and ornithine decarboxylation. +, positive; −, negative.