Table 2.

Comparison of SARS-CoV-2 and BRSV detection using the automated Nanotrap particle and skim milk workflows in wastewater.

Workflow	Sample number	Positive (%)	N1 Ct (SD)	Average N1 GC/μL (SD)‡	BRSV Ct (SD)
Automated Nanotrap with ER1 (10 ml)	29	29 (100)	31.92 (1.60)	50.4 (15.4)	27.92 (0.98)
Skim milk (250 ml)	29	29 (100)	32.97 (1.58)	24.0 (12.3)	34.44 (1.66)

SD, standard deviation.

^‡Average SARS-CoV-2 genome copies per microliter of RNA extracted.

Wilcoxon Signed Rank Test: automated Nanotrap workflow with ER1 vs. skim milk workflow, P = 0.006 (N1) and P < 0.0001(BRSV).