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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: J Periodontol. 2022 Dec 20;94(5):683–693. doi: 10.1002/JPER.22-0462

FIGURE 4.

FIGURE 4

Intracellular protein levels of interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), cyclooxygenase-2 (COX-2), matrix metalloproteinase (MMP)-1, MMP-2, MMP-3, tissue inhibitors of metalloproteinase (TIMP)-1, TIMP-2, and osteoprotegerin (OPG) are produced by periodontal ligament fibroblasts. Under the same treatment conditions, cell lysates were harvested, and the intracellular protein levels of (A) IL-6, (B) IL-8, (C) MCP-1, (D) COX-2, and (J) OPG were assessed by Western blot. The intracellular protein levels of (E) MMP-1, (F) MMP-2, (G) MMP-3, (H) TIMP-1, and (I) TIMP-2 were determined using multiplex immunoassay. Representative images of the Western blot analyses of (K) IL-6, IL-8, MCP-1, and COX-2, and of (L) OPG. Data are presented as mean ± SEM of three independent experiments. COX-2, cyclooxygenase-2; IL, interleukin; MCP, monocyte chemoattractant protein-1; MMP, matrix metalloproteinase; O.D, optical density; OPG, osteoprotegerin; RvD1, resolving D1; TIMP, tissue inhibitors of metalloproteinases. *p < 0.05.