FIG. 2.

Localization of ZipA, FtsA, and FtsZ in wild-type cells. Fluorescence (a through f) and corresponding differential interference contrast (a′ through f′) micrographs showing the location in normally dividing cells of both native (a to c) and Gfp tagged (d to f) ZipA, FtsA, and FtsZ proteins, respectively. Cells shown are from strains PB103 (wild type [wt]) (a to c), CH3(λCH50)/pDB355 [wt(P_lac::zipA-gfp)/cI857 P_λR::ftsA⁺] (d), PB103(λCH75) [wt(P_lac::gfp-ftsA)] (e), and CH3(λDR120)/pDB361 [wt(P_lac::gfp-ftsZ)/cI857 P_λR::zipA⁺] (f). Cells in panels a to c were grown at 37°C prior to immunofluorescence staining with the appropriate antibodies. Cells in panels d to f were grown at 30°C in the presence of 25 μM (d and e) or 50 μM (f) IPTG, and were observed immediately after chemical fixation. Results with a number of other normally dividing lysogens [e.g., PB103(λCH50), CH3(λCH75), and PB103(λDR120)] were similar to those shown in panels d to f. Bar in panel a represents 2.0 μm.