TABLE 2.
Quantitation of cell length and fluorescent rings after HID of FtsZ, FtsA, or ZipAa
| Strain | Depleted protein | Antibody | R− cell result
|
R+ cell result
|
R− + R+ cell result
|
||||
|---|---|---|---|---|---|---|---|---|---|
| % (n) | Avg length (μm) (range) | % (n) | Avg length (μm) (range) | L/R (μm) (range)b | Rings/cell (range) | L/R (μm) | |||
| PB103 | NA | Anti-FtsZ | 29 (21) | 1.5 (1.3–1.8) | 71 (52) | 2.1 (1.4–2.8) | 2.1 (1.4–2.8) | 0.71 (0–1) | 2.7 |
| CH2/pDB280 | FtsA | Anti-FtsZ | 0 (0) | NA | 100 (27) | 29.7 (10.0–55.6) | 6.2 (4.6–10.1) | 4.78 (1–10) | 6.2 |
| CH5/pCH32 | ZipA | Anti-FtsZ | 28 (41) | 45.4 (10.3–75.9) | 72 (103) | 37.7 (10.1–83.2) | 17.2 (3.3–89.5) | 1.56 (0–5) | 25.5 |
| PB103 | NA | Anti-FtsA | 18 (14) | 1.6 (1.3–1.8) | 82 (63) | 2.3 (1.3–3.7) | 2.3 (1.3–3.7) | 0.82 (0–1) | 2.6 |
| PB143/pCX41 | FtsZ | Anti-FtsA | 96 (90) | 14.5 (2.4–39.2) | 4 (4) | 2.9 (2.5–3.7) | 2.9 (2.5–3.7) | 0.04 (0–1) | 329.8 |
| CH5/pCH32 | ZipA | Anti-FtsA | 21 (9) | 40.8 (9.1–65.4) | 79 (34) | 27.6 (8.6–90.8) | 12.0 (5.8–40.8) | 1.81 (0–5) | 16.7 |
| PB103 | NA | Anti-ZipA | 25 (14) | 1.7 (1.3–1.90) | 75 (41) | 2.3 (1.3–3.6) | 2.3 (1.3–3.6) | 0.75 (0–1) | 2.9 |
| PB143/pCX41 | FtsZ | Anti-ZipA | 96 (78) | 17.3 (2.3–53.4) | 4 (3) | 8.1 (2.2–18.8) | 8.1 (2.2–18.8) | 0.04 (0–1) | 457.6 |
| CH2/pDB280 | FtsA | Anti-ZipA | 2 (1) | 16.1 (NA) | 98 (40) | 24.6 (7.1–50.0) | 5.5 (3.9–21.5) | 4.34 (0–10) | 5.6 |
a
Cells were treated and analyzed as described in the text. Some parameters were calculated separately for cells without fluorescent ring structures (R− cells), cells with one or more rings (R+ cells), and all cells combined (R− + R+ cells). NA, not applicable.
b
L/R, ratio of the combined length to the number of rings.