TABLE 3.
Quantitation of cell length and fluorescent rings after CID of FtsZ, FtsA, and ZipAa
| Strain | Depleted protein | Marker | R− cell result
|
R+ cell result
|
R− + R+ cell result
|
||||
|---|---|---|---|---|---|---|---|---|---|
| % (n) | Avg length (μm) (range) | % (n) | Avg length (μm) (range) | L/R (μm) (range) | Rings/cell (range) | L/R (μm) | |||
| PB103(λDR120) | NA | Gfp-FtsZ | 12 (14) | 2.1 (1.7–3.1) | 88 (104) | 2.7 (1.6–4.4) | 2.7 (1.6–4.4) | 0.88 (0–1) | 3.0 |
| CH2(λDR120)/pDB355 | FtsA | Gfp-FtsZ | 7 (4) | 56.1 (18.7–98.9) | 93 (50) | 34.4 (11.9–58.1) | 6.3 (4.7–35.7) | 5.04 (0–11) | 7.2 |
| CH5(λDR120)/pDB361 | ZipA | Gfp-FtsZ | 19 (12) | 36.1 (16.8–63.4) | 81 (52) | 35.5 (5.7–85.5) | 8.6 (4.9–40.2) | 3.36 (0–9) | 10.6 |
| PB103(λCH75) | NA | Gfp-FtsA | 15 (20) | 2.3 (1.6–4.0) | 85 (114) | 2.9 (1.8–6.3) | 2.9 (1.8–6.3) | 0.85 (0–1) | 3.3 |
| PB143(λCH75)/pDB346 | FtsZ | Gfp-FtsA | 100 (56) | 36.7 (5.3–66.3) | 0 (0) | NA | NA | 0 (NA) | >2,054.7 |
| CH5(λCH75)/pDB361 | ZipA | Gfp-FtsA | 8 (7) | 35.4 (15.0–56.3) | 92 (79) | 43.0 (16.8–84.0) | 9.4 (6.1–64.9) | 4.20 (0–9) | 10.1 |
| PB103(λCH50) | NA | ZipA-Gfp | 14 (16) | 2.0 (1.5–3.9) | 86 (100) | 2.7 (1.5–4.2) | 2.7 (1.5–4.2) | 0.86 (0–1) | 3.0 |
| PB143(λCH50)/pDB346 | FtsZ | ZipA-Gfp | 95 (127) | 19.7 (1.9–62.5) | 5 (6) | 10.5 (2.6–26.9) | 10.5 (2.6–26.9) | 0.05 (0–1) | 427.3 |
| CH2(λCH50)/pDB355 | FtsA | ZipA-Gfp | 7 (4) | 32.9 (14.0–55.4) | 93 (50) | 30.7 (10.2–57.9) | 6.4 (3.5–20.0) | 4.43 (0–9) | 7.0 |
a
Cells were treated and analyzed as described in the text. Columns list the same parameters as those described in the footnotes to Table 2 except that the third column (Marker) indicates the Gfp fusion that was used to mark septal rings. NA, not applicable.