Figure 1.

Effect of 1H-imidazo[4,5-c]quinolin-4-amine derivatives on the dissociation of [¹²⁵I]50 using hA₃ARs. HEK-293 membranes stably overexpressing the hA₃AR were incubated with ~0.3 nM [¹²⁵I]50 and 10 μM of the indicated modulator for 3 h. The addition of 100 μM 51 initiated dissociation. The amount of radioligand left remaining after 60 min was measured. Statistical significance was calculated by a two-tailed Student’s t test (n = 3; * denotes P < 0.05). Data are presented as mean ± SEM.