Figure 1.
(A) Photograph of a representative 1.5% nondenaturing agarose gel showing photocleavage of 38 μM bp pUC19 plasmid DNA in the presence of 3 μM N1-(anthracen-9-ylmethyl)ethane-1,2-diaminium dichloride 2 without and with 410 mM of halide salt (10 mM sodium phosphate buffer pH 7.0). The samples in Lanes 1 to 10 were irradiated for 60 min in a ventilated Rayonet photochemical reactor fitted with 8 RPR-3500 Å, 24 W lamps. Abbreviations: L = linear; N = nicked; S = supercoiled. (B) Percentage of cleaved pUC19 (linear DNA + nicked DNA) in the photocleavage reactions averaged over 3 trials. Error bars represent standard deviation.