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. 2023 Jun 22;36(7):1002–1020. doi: 10.1021/acs.chemrestox.2c00235

Figure 2.

Figure 2

Photographs of 1.5% nondenaturing agarose gels showing photocleavage of pUC19 plasmid DNA in the presence of 5 μM N1-(anthracen-9-ylmethyl)ethane-1,2-diaminium dichloride 2 and concentrations of (A) NaCl and (B) KCl ranging from 0 to 400 mM. Samples contained 10 mM sodium phosphate buffer pH 7.0 and 38 μM bp DNA. The reactions in Lanes 1 to 11 were irradiated at 350 nm for 60 min in a ventilated Rayonet photochemical reactor fitted with 8 RPR-3500 Å, 24 W lamps. In Lanes 10 and 11 of (B), the plasmid was photodegraded into a diffuse band of low-molecular-weight DNA fragments.30 ,31 Percent cleavage yields reflect linear, nicked, and fragmented DNA. Abbreviations: L = linear; N = nicked; S = supercoiled.