Figure 3.
Photographs of 1.5% nondenaturing agarose gels showing photocleavage of pUC19 plasmid DNA in the presence and absence of 5 μM N1-(anthracen-9-ylmethyl)ethane-1,2-diaminium dichloride 2 and 0 to 400 mM of (A) NaF, (B) KF, (C) NaBr, (D) KBr, (E) NaI, and (F) KI. Samples contained 10 mM sodium phosphate buffer pH 7.0 and 38 μM bp DNA. The reactions in Lanes 1 to 11 were irradiated at 350 nm for 60 min. Percent cleavage yields reflect linear and nicked DNA. Abbreviations: L = linear; N = nicked; S = supercoiled.