Table 1. Average % Change in DNA Photocleavage Induced by Scavengers and D2Oae.
reagents (target) | EDTA (Mn+) | Na benzoate (•OH) | Tiron (O2•–) | D2O (1O2) | catalase (H2O2) |
---|---|---|---|---|---|
2 no-saltb | –52 ± 8 | –30 ± 13 | –61 ± 4 | –1 ± 1 | +6 ± 6 |
2 saltb | –46 ± 7 | –40 ± 4 | –74 ± 4 | +4 ± 4 | +2 ± 2 |
2 no-saltc | nd | nd | nd | nd | +10 ± 1 |
2 saltc | nd | nd | nd | nd | –26 ± 4 |
2 no-saltd | nd | –48 ± 6 | nd | –21 ± 3 | +13 ± 8 |
2 saltd | nd | –85 ± 3 | nd | +1 ± 2 | –68 ± 3 |
Photocleavage reactions consisted of 38 μM bp of pUC19 plasmid DNA equilibrated with 10 mM sodium phosphate buffer pH 7.0.
10 μM2, and either 100 mM EDTA or Na benzoate, 10 mM Tiron, 100 U/μL of catalase, or D2O (72% final volume) in the absence and presence of 400 mM KCl.
2.5 μM2 and 100 U/μL of catalase in the absence and presence of 400 mM KCl.
1 μM2, and either 100 mM scavenger, 100 U/μL of catalase, or D2O in the absence and presence of 150 mM NaCl and 260 mM KCl.
b–dSamples were aerobically irradiated for 60 min at 350 nm. Data were averaged over three to five trials with error reported as standard deviation.dData in row previously published.30