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. 2023 Jun 22;36(7):1002–1020. doi: 10.1021/acs.chemrestox.2c00235

Table 1. Average % Change in DNA Photocleavage Induced by Scavengers and D2Oae.

reagents (target) EDTA (Mn+) Na benzoate (OH) Tiron (O2•–) D2O (1O2) catalase (H2O2)
2 no-saltb –52 ± 8 –30 ± 13 –61 ± 4 –1 ± 1 +6 ± 6
2 saltb –46 ± 7 –40 ± 4 –74 ± 4 +4 ± 4 +2 ± 2
2 no-saltc nd nd nd nd +10 ± 1
2 saltc nd nd nd nd –26 ± 4
2 no-saltd nd –48 ± 6 nd –21 ± 3 +13 ± 8
2 saltd nd –85 ± 3 nd +1 ± 2 –68 ± 3
a

Photocleavage reactions consisted of 38 μM bp of pUC19 plasmid DNA equilibrated with 10 mM sodium phosphate buffer pH 7.0.

b

10 μM2, and either 100 mM EDTA or Na benzoate, 10 mM Tiron, 100 U/μL of catalase, or D2O (72% final volume) in the absence and presence of 400 mM KCl.

c

2.5 μM2 and 100 U/μL of catalase in the absence and presence of 400 mM KCl.

d

1 μM2, and either 100 mM scavenger, 100 U/μL of catalase, or D2O in the absence and presence of 150 mM NaCl and 260 mM KCl.

e

b–dSamples were aerobically irradiated for 60 min at 350 nm. Data were averaged over three to five trials with error reported as standard deviation.dData in row previously published.30