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Analysis of peptidoglycan composition by RP-HPLC. Samples of peptidoglycan isolated from M600 (sigE+) (A) or J2130 (ΔsigE) (B) were digested with Cellosyl and subjected to HPLC analysis, and the A202 values of the eluates were monitored. Muropeptides that show particularly different abundances in the two strains are marked (X1 through X3).
