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The ΔsigE mutant, J2130, overproduces the blue-pigmented antibiotic actinorhodin on medium deficient in Mg2+. Ten microliters of a spore suspension (108 spores ml−1) was spotted on L agar alone or on L agar plus 1.6 mM MgSO4. Plates were photographed after 3 days of incubation at 30°C.
