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. 2023 Jul 18;16:116. doi: 10.1186/s13068-023-02367-3

Table 3.

KPI of a pulsed fed-batch process with C. glutamicum ΔfasR cg2692TTG CgLP12::(Ptac-pntAB-TrrnB) gX (pEKEx2-maqu2220) with wheat straw hydrolysate

Phase KPI
Batch phase QP|g L−1 h−1 0.049 ± 0.001
YP/S|Cmol Cmol−1 0.038 ± 0.002
Fed-batch phase QP|g L−1 h−1 0.227 ± 0.028
YP/S|Cmol Cmol−1 0.065 ± 0.008
Total QP|g L−1 h−1 0.109 ± 0.005
YP/S|Cmol Cmol−1 0.054 ± 0.005
CDWmax|gX L−1 37.4 ± 1.1
cFAL,max|g L−1 2.45 ± 0.09

The carbon source during the batch phase was provided by hydrolysate, normalized to a concentration of 40 g glucose L−1. 30-mL pulses of a 350 g glucose L−1 hydrolysate stock solution were added three times upon a sharp increase of the DO signal. Data represent means of 3 biological replicates ± standard deviations