Table 3.
KPI of a pulsed fed-batch process with C. glutamicum ΔfasR cg2692TTG CgLP12::(Ptac-pntAB-TrrnB) gX (pEKEx2-maqu2220) with wheat straw hydrolysate
| Phase | KPI | |
|---|---|---|
| Batch phase | QP|g L−1 h−1 | 0.049 ± 0.001 |
| YP/S|Cmol Cmol−1 | 0.038 ± 0.002 | |
| Fed-batch phase | QP|g L−1 h−1 | 0.227 ± 0.028 |
| YP/S|Cmol Cmol−1 | 0.065 ± 0.008 | |
| Total | QP|g L−1 h−1 | 0.109 ± 0.005 |
| YP/S|Cmol Cmol−1 | 0.054 ± 0.005 | |
| CDWmax|gX L−1 | 37.4 ± 1.1 | |
| cFAL,max|g L−1 | 2.45 ± 0.09 | |
The carbon source during the batch phase was provided by hydrolysate, normalized to a concentration of 40 g glucose L−1. 30-mL pulses of a 350 g glucose L−1 hydrolysate stock solution were added three times upon a sharp increase of the DO signal. Data represent means of 3 biological replicates ± standard deviations