TABLE 2.
Sequence of cloned segment (bp) | Allele | Amino acid change(s) in gpA | PFU/viable cell |
---|---|---|---|
1818–2556 | A+ | None | <5.0 × 10−6 |
458–5548 | A-E515G | E515G | 1.1 × 10−1 |
2212–2847 | A-N509K | N509K | 4.1 × 10−3 |
2190–2556 | A-R504C | R504C | 7.7 × 10−4b |
1818–2556 | A-R504C | P416S, R504C | 1.9 × 10−3b |
1818–2190 | P416S | <5.3 × 10−5b |
Crosses were performed with plasmids in an MF1427 (λ cosN G2C C11G gal+ att+ cI857) lysogen and were plated on MF1972 for plaques. The higher frequency of recombinants for A-E515G is a reflection of the larger λ DNA insert in the plasmid used (5 kb).
These lysates were plated on MF1427 for recombinants forming normal-sized plaques.