TABLE 4.
Burst sizes of phages with various cosN and A alleles normalized to λ cosN+ A+a
| Genotype | Burst size (PFU/cell) in:
|
|
|---|---|---|
| MF1427 | MF1972 | |
| cosN+ A+ | 1.0 | 0.29 |
| cosN G2C C11G A+ | 0.01b | 8.2 × 10−4 |
| cosN G2C C11G A-E515G | 0.83 | 0.18 |
| cosN G2C C11G A-N509K | 0.69 | 0.03 |
| cosN G2C C11G A-R504C | 0.43 | 0.07 |
| cosN+ A-E515G | 1.01 | 0.23 |
| cosN+ A-N509K | 0.60 | 0.29 |
| cosN+ A-R504C | 0.38 | 0.25 |
λ-P1:5R Knr cI857 lysogens in MF1427 (IHF+) and MF1972 (IHF−) were induced and plated on MF1427. Values are PFU/induced cell, normalized to λ cosN+ A+ induced from MF1427, and are the averages of three experiments. The average burst of λ cosN+ A+ on MF1427 was 243 and that on MF1972 was 71.6.
These lysates were plated on MF2548 to reduce the background of revertants which arise spontaneously on MF1427.