TABLE 5.
Effect of changing gpA residue 515 on viability of λ cosN+ and λ cosN G2C C11Ga
| Group | Amino acid | Viable recombinants on MF1972 for:
|
|
|---|---|---|---|
| cosN+ | cosN G2C C11G | ||
| Acidic | D | + | − |
| E | + | − | |
| Basic | K | + | − |
| R | + | − | |
| Polar uncharged | Q | + | + |
| S | + | + | |
| Hydrophobic | A | + | + |
| F | + | + | |
| G | + | + | |
| I | + | + | |
| V | + | + | |
| Y | + | + | |
Crosses were performed in an MF1966 (λ Aam515 cI857) lysogen for the cosN+ allele and in an MF1427 (λ cosN G2C C11G gal+ att+ cI857) lysogen for the cosN G2C C11G allele; both were plated on MF1972 for plaques.