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. 2023 Jun 26;14(10):1920–1934. doi: 10.7150/jca.85032

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A. Total protein expression of Cdc42, PAK1, PKA1α, cathepsin B, cathepsin D, PTPN14, and STMN1 in LoVo (a) and Hct116 (b) control and CoCl₂-treated cells before and after ML141 treatment. B. Clonal formation of CoCl₂-treated LoVo and Hct116 before and after ML141 treatment. C. Cell invasion assay of CoCl₂-treated LoVo and Hct116 cells before and after ML141 treatment (40×). D. Cell migration experiments of LoVo and Hct116 CoCl₂-treated cells before and after ML141 treatment (40×). E. Histograms of the mean cell numbers of CoCl₂-treated LoVo and Hct116 cells in clonogenic efficiency (a), invasion (b), and migration (c) experiments before and after ML141 treatment. F. Expression of PAK1 after transfection with siRNA PAK1-2133, 1228, 629, PC, NC, and MC in LoVo control (a), CoCl₂-treated LoVo cells (b), Hct116 cells (c), and CoCl₂-treated Hct116 cells (d). CTR, control cells; TRE, CoCl₂-treated cells; PC, positive control; NC, negative control; MC, mock control.