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. 2023 Jun 26;14(10):1920–1934. doi: 10.7150/jca.85032

Figure 6.

Figure 6

A. (a,b) Total protein expression of cathepsin B, cathepsin D, α/β tubulin, PTPN14, STMN1, and P38MAPK after the knockdown of PAK1 expression in LoVo control and CoCl2-treated cells. B. (a,b) Total protein expression of cathepsin B, cathepsin D, α/β tubulin, PTPN14, STMN1, and P38MAPK after the knockdown of PAK1 expression in Hct116 control and CoCl2-treated cells. C. (a, b) Expression of four phosphorylation sites of STMN1 in CoCl2-treated LoVo and Hct116 cells transfected with siRNA PAK1-2133, PC, NC, and MC, respectively. (c, d) The nuclear expression of four phosphorylation sites of STMN1 in LoVo and Hct116 CoCl2-treated cells transfected with siRNA PAK1-2133, PC, NC, and MC, respectively. D. Verification of the interaction between PAK1 and P-STMN1 by Co-IP in LoVo control and CoCl2-treated cells (a) and Hct116 control and CoCl2-treated cells (b). E. (a, b) Total protein expression of PAK1, P38MAPK, STMN1, and α/β tubulin after transfection with PTPN14 overexpression plasmid in CoCl2-treated LoVo and Hct116 cells. CTR, control cells; TRE, CoCl2-treated cells; PC, positive control; NC, negative control; MC, mock control.