Fig. 3.

The influence of knocking down PELP1 on E₂ and E₂-BSA-induced MMP-9 expression in MCF-7. Either PELP1-shRNA or non-specific control (NC) plasmid was transfected into MCF-7, after selected with puromycin, the protein expression of PELP1 was examined by Western blot (a). MFC-7 transfected with NC or PELP1-shRNA were treated with 10 nM of either E₂ or E₂-BSA; the mRNA (b) and protein expression (c) of MMP-9 were detected respectively by real-time PCR and Western blot. All presented figures are representative data from at least three independent experiments. *P < 0.05 compared with NC and medium treated control; ^#P < 0.05 compared with that NC treated with 10 nM E₂; ^&P < 0.05 compared with that NC treated with 10 nM E₂-BSA