TABLE 2.
Characterization of strains 64H, KB6417, and KB6419
| Strain | Activity
|
Doubling
timea (h)
|
||||
|---|---|---|---|---|---|---|
| Transportb (nmol/min/5 ×
108 cells)
|
Galactokinase (nmol/mg of protein/min) | |||||
| Galactose | Lactose | Galactose | Lactose | Glucose | ||
| 64H | 3.4 | 3.5 | 150 | 2.2 | 2.2 | 1.8 |
| KB6417 | 0 | 0 | 240 | 3.2 | ∞ | 3.2 |
| KB6419 | 0 | 0 | 413 | 3.4 | ∞ | 3.4 |
Determined by measuring the OD420 of cultures growing in CTM supplemented with 0.5% carbohydrate.
Measured with 7.5 μM [14C]galactose or 15 μM [14C]lactose.