Analysis of FCR1 expression in the C. albicans transformants CAI4[pVEC] (lane 1), FM7[pVEC] (lane 2), FM7[pVEC/FCR1] (lane 3), CAI4[YPB-ADH] (lane 4), FM7[YPB-ADH] (lane 5), and FM7[YPB-ADH/FCR1] (lane 6). Total RNA samples (lanes 1 to 5 contained 20 μg, and lane 6 contained 0.4 μg) were electrophoresed on an agarose gel and transferred to a nylon membrane. The membrane was hybridized simultaneously with an FCR1 probe and an ACT1 probe. Autoradiography was for 36 h, with two intensifying screens at −80°C.