FIG. 7.

Cleavage of an oligonucleotide containing a unique abasic site. Oligonucleotide (100 fmol) was incubated for 10 min at 32°C with enzyme. The products of the reaction were separated by SDS-PAGE, and the results were quantified with PhosphorImager. (A) Increasing amounts of fraction V (■) and fraction VI (▴). (B) Lane 1, duplex oligonucleotide containing an AP site. lane 2, like lane 1 but incubated with 0.2 M NaOH before analysis; lane 3, like lane 1 but incubated with 10 ng of E. coli Fpg protein; lane 4, like lane 1 but incubated with 10 ng of E. coli Nth protein; lane 5, like lane I but incubated with 10 ng of fraction V enzyme; lane 6, like lane 1 but incubated with 10 ng of fraction VI enzyme.