TABLE 1.
Purification of D. radiodurans Fapy-DNA glycosylase activities
| Step | Fraction | Protein (mg) | Fapy-DNA glycosylase sp act (U/mg)a | Yield (%) | Purification factor (fold) |
|---|---|---|---|---|---|
| Crude extract | I | 6,256 | 0.6 | 100 | 1 |
| QMA-Acell | II | 4,955 | 0.6 | 94 | 1 |
| Phospho-Ultrogel | III | 857 | 8.2 | 64 | 13 |
| Phenyl Superose-FPLC | IVa | 39.5 | 6.8 | 7 | 11.3 |
| IVb | 35 | 24 | 22 | 40 | |
| MonoS FPLC | |||||
| Fraction IVa | V | 0.5 | 182 | 2.4 | 300 |
| Fraction IVb | VI | 0.31 | 1,210 | 10 | 2,000 |
a
Measured with [3H]Fapy–poly(dG-dC) as the substrate. One unit excises I pmol of Fapy residues in 5 min at 32°C.