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Kinetic parameters for excision of Fapy and 8-oxoG (from oligonucleotides) residues for D. radiodurans fraction V and fraction VI enzymes
| Protein | Fapy-DNA glycosylase activity
|
8-oxoG-DNA glycosylase activity
|
||||
|---|---|---|---|---|---|---|
| Km (nM) | kcat (1/min) | kcat/Km (1/nM · min) | Km (nM) | kcat (1/min) | kcat/Km (1/nM · min) | |
| Fraction V | 22.1 | 0.33 | 0.16 | 83.3 | 0.2 | 0.0024 |
| Fraction VI | 5.3 | 0.49 | 0.1 | 10 | 0.6 | 0.06 |
| E. coli Fpg protein | 10 | 0.5 | 0.05 | 4 | 0.43 | 0.11 |
