Fig. 3.

Effect of HET0016 on DHT-induced viability in LNCaP cells. Cells were grown in steroid-deprived media for 48 h before the addition of DHT and/or HET0016, and cultures were continued in the specified conditions for another 5 days. Cell viability was assessed by the MTT assay, and results are expressed as percentage of control cells (vehicle-treated cells or vehicle + HET0016-treated cells) (n = 3–4, in sextuplicates) (ANOVA p < 0.0001); (***p < 0.001 vs. vehicle; ##p < 0.01 vs. vehicle + HET0016, 10 μM; ###p < 0.001; +++p < 0.001 vs. DHT 0.1 nM). Values are presented as the mean ± SEM