Fig. 4.

Effect of 20-HETE on the viability of prostate cancer cells. Cells were grown in steroid-deprived (a, b) or complete media (c, d) for 48 h before the addition of the indicated concentrations of 20-HETE plus indomethacin (10 μM), and cultures were continued for another 5 days. Cell viability was assessed by the MTT assay. Results are expressed as percentage of control cells (vehicle plus indomethacin-treated cells). a n = 3, in sextuplicates (ANOVA p < 0.0001; *p < 0.05; **p < 0.01; ***p < 0.001 vs. control). b n = 3–4, in sextuplicates (ANOVA p = 0.3543). c (n = 2, in sextuplicates) (ANOVA p < 0.0001; ****p < 0.0001 vs. control). d n = 2, in sextuplicates (ANOVA p < 0.0001; ****p < 0.0001 vs. control). Values are presented as the mean ± SEM