Fig. 5.

Effect of HET0016 on the proliferation of LNCaP cells. Cells synchronized by serum deprivation for 16 h were further grown for another 48 h in a complete media in the presence of vehicle (control) or HET0016 or b steroid-deprived media in the presence of vehicle (control) or the indicated drugs. The expression of the nuclear Ki-67 antigen was analyzed by immunocytochemistry, and quantitation of cell proliferation is expressed as percentage of Ki-67+ cells. a n = 2, in triplicates (ANOVA p = 0.0045; **p < 0.01 vs. vehicle). b n = 3, in triplicates (ANOVA p = 0.0078; *p < 0.05 vs. vehicle; +p < 0.05 vs. DHT 0.1 nM). Values are presented as the mean ± SEM