Fig. 9.

Role of 20-HETE in the regulation of the expression of the androgen receptor in LNCaP cells. LNCaP cells were grown in complete media for 8, 24, or 48 h in the presence of vehicle (control) and HET0016, 1 or 10 μM. Cells were collected and subjected to RNA and protein extractions for the quantitation of androgen receptor (AR) mRNA normalized to HPRT-1 (qPCR) (a) and the analysis of AR protein expression normalized to β-tubulin (b). The expression levels were calculated as the values relative to that of a calibrator sample (control, 8 h). Culture supernatants were collected for the measurement of secreted prostate-specific antigen (PSA) (c); results are presented as nanograms of PSA every 1 × 10⁵ cells. a n = 2–3, in duplicates (8 h: ANOVA p = 0.0183; *p < 0.05 vs. vehicle; 24 h: ANOVA p = 0.5870). b n = 2 (8 h: ANOVA p < 0.0001; ***p < 0.001 vs. vehicle; 24 h: ANOVA p < 0.0001; **p < 0.01; ***p < 0.001 vs. vehicle; 48 h: ANOVA p = 0.0008; ***p < 0.001 vs. vehicle). c n = 2, in duplicates (8 h: ANOVA p = 0.4648; 24 h: ANOVA p = 0.0006; **p < 0.01; ***p < 0.001 vs. vehicle; 48 h: ANOVA p = 0.0002; ***p < 0.001 vs. vehicle). Values are presented as the mean ± SEM