Table 2.

Intracellular levels of 20-HETE in prostate cancer cell lines

Cell line	20-HETE (ng/mg protein)
	Control	HET0016
		1 μM	10 μM
LNCaP	312.8 ± 62.0	139.0 ± 28.9*	22.97 ± 0.4**
PC-3	207.3 ± 9.0	55.1 ± 1.0*	N.D.

Effect of HET0016. Cells grown in complete media were incubated for 6 h with vehicle (control) or the indicated concentrations of HET0016. Cell homogenates were analyzed for 20-HETE by ELISA. LNCaP cells n = 2 in duplicates, ANOVA p = 0.0002 followed by Dunnet. PC-3 cells n = 2 in duplicates, t test p < 0.0001. Values are mean ± SEM

N.D. not determined

*p < 0.01; **p < 0.01 vs. control