Table 2.
Estrous cycle analysis was performed just prior to collection of mice at 12 months of age
| Genotype (no. of mice) | % cycling | Days to complete a cycle | Days spent in stage(s) over estrous cycle | |||||
|---|---|---|---|---|---|---|---|---|
| Proestrus | Estrus | Proestrus + estrus | Metestrus | Diestrus | Metestrus + diestrus | |||
| C (N = 11) | 63.6 | 4.3 ± 0.2 | 1.0 ± 0.0 | 1.1 ± 0.1 | 2.1 ± 0.1 | 1.1 ± 0.1 | 1.0 ± 0.0 | 2.1 ± 0.1 |
| B (N = 9) | 66.7 | 5.5 ± 0.3* | 1.5 ± 0.2* | 1.0 ± 0.3 | 2.5 ± 0.3 | 1.5 ± 0.2 | 1.5 ± 0.3 | 3.0 ± 0.4* |
| BT (N = 11) | 63.6 | 4.3 ± 0.2 | 1.1 ± 0.1 | 1.1 ± 0.1 | 2.3 ± 0.2 | 1.0 ± 0.0 | 1.0 ± 0.0 | 2.0 ± 0.0 |
| F (N = 10) | 80.0 | 4.6 ± 0.3 | 1.0 ± 0.0 | 1.3 ± 0.2 | 2.3 ± 0.2 | 1.3 ± 0.2 | 1.1 ± 0.1 | 2.4 ± 0.2 |
| BF (N = 10) | 60.0 | 5.2 ± 0.3* | 1.2 ± 0.2 | 1.2 ± 0.2 | 2.3 ± 0.2 | 1.5 ± 0.2 | 1.3 ± 0.2 | 2.8 ± 0.2* |
| BTF (N = 9) | 66.7 | 5.0 ± 0.3 | 1.3 ± 0.2 | 1.2 ± 0.2 | 2.5 ± 0.2 | 1.3 ± 0.2 | 1.2 ± 0.2 | 2.5 ± 0.2 |
Follicular Brca1 GC−/− mutation alone or combined with Trp53 +/− and/or the presence of Tg.FSH had no effect on the ability of females to exhibit estrous cycling. Proestrus and cycle length were significantly longer in B versus C mice (one-way ANOVA, p < 0.05). Overall, there was no significant B genotype effect on estrous cycle or stages using all groups containing Brca1 GC−/− (two-way ANOVA, p = 0.06). Combined preovulatory stages (proestrus + estrus) were similar in all groups. Diestrus stage length was also equivalent in all groups. Combined post-ovulatory (metestrus + diestrus) stage times were longer in the B and BF groups (one-way ANOVA p < 0.05). *p < 0.05, one-way ANOVA