Fig. 3.
The effects of STAT3 inhibitor, S3I-201, in HFD-treated obese ThrbPV/PVPten+/− mice. a Survival curves for HFD-ThrbPV/PVPten+/− mice treated with S3I-201 or vehicle. Intraperitoneal injection three times a week from 8 weeks of age until they had to be euthanized because of sickness. Data are presented by Kaplan-Meier methods and analyzed by log-rank test. The p values are indicated. b Thyroid weights of vehicle-treated or S3I-201-treated wild-type or ThrbPV/PVPten+/−. c Western blot analysis of protein abundance of leptin receptor, phosphorylated -JAK2 (Y1007/1008), total JAK-2, phosphorylated STAT3 (Y705), total-STAT3, and GAPDH as a loading control after treatment with vehicle or S3I-201 in wild-type and ThrbPV/PVPten+/− mice. d Representative examples of hematoxylin and eosin (H&E)-stained thyroid sections from wild-type mice treated with vehicle (panel a) and S3I-201 (panel b) and lung sections treated with vehicle (panel a′) and S3I-201 (panel b′) and thyroid tumor sections from ThrbPV/PVPten+/− treated with vehicle (panel c) and S3I-201 (panel d) and lung sections treated with vehicle (panel c′) and S3I-201 (panel d′). The arrows indicate vascular invasion (panel d) and anaplasia (panel c). [36]