Fig. 3.

a–e Effects of MIG6 silencing on cell viability in Ishikawa (a) and HEC1B (b) cells and those of MIG6 overexpression in Ishikawa (c), HEC1B (d), and AN3CA (e) cells. The WST-1 assay showed that the knockdown of MIG6 increased and with its transfection decreased the viability of these cells. f Effects of MIG6 silencing on MPA responses. MIG6 silencing decreased the MPA-induced reduction of PR-transfected Ishikawa cell viability. The WST-1 assay was performed after 72 h of incubation. P or Parent parent cells without transfection, si-MIG6 MIG6 siRNA-transfected cells, Sc scrambled negative control siRNA transfected cells, mock empty vector-transfected cells, MIG6 MIG6 cDNA-transfected cells. Asterisk: significant difference (P < 0.01) compared with Sc or mock