Fig. 5.

a, b Effects of MPA and LBH589 on the survival of HEC1B cells (a) and its graphical demonstration (b). Fluorescent staining was performed at 48 h after the addition of LBH589 and/or MPA. Middle panel: Annexin V (green). Lower panel: PI (red). MPA with LBH589 increased the number of apoptotic cells. c Effects of MPA and LBH589 on the cell cycle. MPA with LBH589 increased the sub-G1 population. Flow cytometry was performed at 48 h after the addition of LBH589 and/or MPA. d, e Effects of MIG6 silencing on the LBH589-induced sub-G1 population (d) and viability reduction (e). The numerical values were acquired by comparing with NT. MIG6 silencing eliminated the LBH589-mediated sub-G1 population and viability reduction. NT no treatment, Combination treated with LBH589 and MPA, si-MIG6 (A) MIG6 siRNA (A) transfected cells, Sc scrambled negative control siRNA transfected cells. Asterisk: significant difference (P < 0.05) compared with NT or Sc. Dagger: significant difference (P < 0.05) compared with LBH589