Fig. 2.

WT versus N-terminus variants of the AIP protein on cAMP-driven transcription. Luciferase reporter activity of GH3 cells transfected with either empty vector (EV), wild-type (WT) or N-terminus variant AIP (R9Q, R16H, V49M and K103R expression vectors) and rat Rip1 promoter-driven luciferase expression plasmid. Treatment with 10 μM IBMX and/or 1 μM forskolin 48 h after transfection is described below. Bars represent means of five separate experiments with three replicates per experiment. Error bars indicate standard error. (*p = <0.05, **p = <0.005; For, forskolin)