Fig. 6.

Cycloheximide chase analysis of WT and N-terminus variants of the AIP proteins. GH3 cells were transfected with WT or N-terminus variants of the AIP protein (R9Q, R16H, V49M and K103R), and cycloheximide was added 48 h after transfection. Cells were lysed at 0, 20, 24 and 28 h after addition of cycloheximide. a Western blot analysis of GH3 cell lysates with AIP (lower panel) and β-actin (upper panel) antibodies for the detection of endogenous and over-expressed AIP and its degradation at different time points from the cessation of translation by cycloheximide. b Degradation rate of WT and N-terminus variants of the AIP at different time points indicates a variation in half-lives. Each point represents the mean of five separate experiments. Error bars indicate standard error. (*p = <0.05) (C control; GH3 cells transfected with empty vector after 48 h)