Fig. 5.
Overexpression of IGF1R partially attenuated miR-15b-inhibited cell growth. a The protein expression level of IGF1R in U87 cells was detected using western blot. Then, the mRNA expression level of IGF1R in U87 cells was detected using qRT-PCR assay. b The U87 and U251 cell growth was detected using CCK-8 assay. Cells with miR-15b or miR-NC were transfected with IGF1R plasmids or vector control, respectively. c The cell cycle was detected using flow cytometry. *p < 0.001 vs control