Fig. 6.

miR-15b affected the U87-engrafted tumor growth. U87 cells stably transfected with miR-15b-expressing plasmids or empty vector were subcutaneously injected into nude mice (three nude mice for the experiment group, and three nude mice for the control group), and tumor volumes were measured every week. Tumors were resected and weighed at 4 weeks after cell injection. Each injection contained 40 ng of miR-15b mimic in 10 μL saline solution. Mice were stitched and reanimated under warm light. Control mice were injected with mimic control. a The expression of miR-15b was detected using qRT-PCR assay. b The expression of IGF1R mRNA was detected using qRT-PCR assay. c We identified that miR-15b mimics could repress the U87-engrafted tumor growth when compared with control-induced U87-engrafted tumors. The weight of miR-15b-treated tumors was obviously lower compared with control-treated U87-engrafted solid tumor mass. d The expression of IGF1R protein detected using western blot. *p < 0.001 vs control