Fig. 6.

T3 modulates breast cancer cell adhesion and migration through Src/FAK/PI3K pathway. Cells were treated with T3 (1 nM) for 48 h in the presence or absence of the Src inhibitor (PP2; 0.2 μM), the PI3kinase inhibitor wortmannin (WM; 30 nM), the FAK inhibitor (FAKi 1 μM), and MAP Kinase inhibitor PD98059 (PD 5 mM). a, b The arrows indicate the direction of migration. The upper black lines indicate the starting line and the lower black lines the mean migration distance. c, d Representative images of T-47D cell adhesion to gelatin after T3 treatment (1 nM/2 h) are shown. Experiments were performed in triplicate; representative images are shown