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. 1999 Jan;181(1):284–290. doi: 10.1128/jb.181.1.284-290.1999

FIG. 2.

FIG. 2

Alignment of the amino acid sequences for family 12 endoglucanases in the region of the catalytic nucleophile (†) and acid-base (‡). Residues that occur in at least 10 of the 14 sequences are in boldface. The numbers refer to the amino acids in the following proteins (accession numbers, where available, are given in parentheses): pf eglA, P. furiosus endoglucanase; tm celA, T. maritima endoglucanase A (Z69341); tm celB, T. maritima endoglucanase B (Z69341); tn celA, T. neapolitana endoglucanase A (U93354); tn celB, T. neapolitana endoglucanase A (U93354); rt celA, R. marinus cellulase (U72637); ak celA, Aspergillus kawachii endoglucanase A (D12901); ao cel, Aspergillus oryzae endoglucanase (D83731); aa gun, Aspergillus aculeatus endoglucanase (P22669); tr gun, Trichoderma reesei endoglucanase (AB003694); sr, eglS, S. rochei endoglucanase S (X73953); sl celB, S. lividans cellulase B (U04629); sh celA2, Streptomyces halstedii cellulase A2 (U51222); ec celS, E. carotovora cellulase S (P16630); mt rv1090, Mycobacterium tuberculosis putative endoglucanase (AL021897). The percentages to the right indicate the amino acid sequence identities between the indicated enzyme and the P. furiosus endoglucanase as determined by using BESTFIT (gap creation penalty, 1; gap extension penalty, 0.3).