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. 2023 Jun 15;12(12):e028298. doi: 10.1161/JAHA.122.028298

Figure 3. Prolylcarboxylpeptidase localizes in lysosomes and mediates intracardiac angiotensin II degradation through the lysosomal pathway.

Figure 3

A, Immunostaining of prolylcarboxylpeptidase (red) and Lamp1 (lysosome marker) in H9C2 cells shows prolylcarboxylpeptidase partially resides in lysosomes. White arrows indicate colocalization of prolylcarboxylpeptidase and Lamp1 (yellow dots) (scale bar: 15 μm). B, Cytosolic and lysosomal fractions of wild‐type hearts show the localization of prolylcarboxylpeptidase in both cytosol and lysosome. Lamp1 is used as a marker for lysosomes. C, Angiotensin II levels in plasma. D, Angiotensin 1‐7 levels in plasma. E, Angiotensin II levels in myocardial tissues. F, Angiotensin 1‐7 levels in myocardial tissues (n=8 mice per group). Data are expressed as means±SEM. Two‐way ANOVA with Bonferroni correction for post hoc comparisons was used for analysis. AngII indicates angiotensin II; DAPI, 4′, 6‐diamidino‐2‐phenylindole; Lamp1, lysosomal‐associated membrane protein 1; PRCP, prolylcarboxylpeptidase; and PRCP‐KO, prolylcarboxylpeptidase knockout.