Table 1.
Sheep IgG, mouse IgG and C4d deposition in glomeruli
|
NTS+platelet depletion |
NTS+DEX |
NTS+Anti-IL6 |
NTS+Anti-TGFβ |
NTS+Anti-TPO |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Isotype |
Platelet Depletion |
P Value |
Controla |
DEX |
P Value |
Controla |
Anti-IL6 |
P Value |
Isotype |
Anti-TGFβ |
P Value |
Isotype |
Anti-TPO |
P Value |
|
|
Sheep IgG (MFI per glomerulus) |
88.55±5.20 |
82.32±6.92 |
0.2740 |
85.01±6.29 |
91.13±11.05 |
0.8392 |
85.01±6.29 |
75.98±8.99 |
0.5135 |
73.98±3.98 |
77.39±5.16 |
0.655 |
101.9±8.91 |
103.2±8.63 |
>0.999 |
|
Mouse IgG (MFI per glomerulus) |
64.38±5.35 |
73.09±7.70 |
0.3217 |
125.7±11.80 |
108.1±11.24 |
0.5395 |
125.7±11.8 |
72.98±6.32 |
0.002b |
64.66±7.32 |
66.99±6.63 |
0.717 |
87.87±4.70 |
94.72±4.68 |
0.287 |
|
C4d (MFI per glomerulus) |
55.67±5.27 |
54±5 |
>0.999 |
85.97±5.26 |
37.02±5.66 |
0.002b |
85.97±5.26 |
57.82±11.89 |
0.076 |
45.58±5.72 |
42.55±3.69 |
0.657 |
48.87±6.80 |
50.22±5.80 |
0.959 |
Data represent mean± SEM. IgG, Immunoglobulin G; NTS, nephrotoxic serum; DEX, dexamethasone treatment; TPO, thrombopoietin; MFI, mean fluorescence intensity.
The control group is composed of Phosphate-buffered saline-treated and isotype-treated mice, pooled from two experiments.
P < 0.01, NTS+treatment versus NTS control group, Mann–Whitney test.